Uroporphyrinogen III decarboxylase
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| UroD drawn from PDB 1URO. | |
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uroporphyrinogen decarboxylase
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| Identifiers | |
| Symbol(s) | UROD |
| Entrez | 7389 |
| OMIM | 176100 |
| RefSeq | NM_000374 |
| UniProt | P06132 |
| Other data | |
| EC number | 4.1.1.37 |
| Locus | Chr. 1 p34 |
Uroporphyrinogen III decarboxylase (UroD) is a homodimeric enzyme (EC 4.1.1.37, PDB 1URO) which catalyzes the fifth step in heme biosynthesis: the elimination of carboxyl groups from the four acetate side chains of uroporphyrinogen III to yield coproporphyrinogen III.
At low substrate concentrations the reaction is believed to follow an ordered route, with the sequential removal of CO2 from the D, A, B, and C rings, whereas at higher substrate/enzyme levels a random route seems to be operative. The enzyme functions as a dimer in solution, and both the enzymes from human and tobacco have been crystallized and solved at good resolutions.
UroD is regarded as an unusual decarboxylase, since it performs decarboxylations without the intervention of any cofactors, unlike the vast majority of decarboxylases. Its mechanism has recently been proposed to proceed through substrate protonation by an arginine residue.
Silva & Ramos (2005) performed a detailed study of competing reaction mechanisms proposed for uroporphyrinogen decarboxylase.
[edit] Reference
- Silva PJ, Ramos MJ. Density-functional study of mechanisms for the cofactor-free decarboxylation performed by uroporphyrinogen III decarboxylase. J Phys Chem B 2005;109:18195-200. DOI:10.1021/jp051792s.
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