Spiroplasma
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Spiroplasma is a genus of Mollicutes, a group of small bacteria without cell walls. Spiroplasma shares the simple metabolism, parasitic lifestyle, fried-egg colony morphology and small genome of other Mollicutes, but has a distinctive helical morphology, unlike Mycoplasma. Most spiroplasmas are found either in the gut or hemolymph of insects, or in the phloem of plants. Spiroplasmas are fastidious organisms, which require a rich culture medium. Typically they grow well at 30°C, but not at 37°C. A few species, notably Spiroplasma mirum, grow well at 37°C (human body temperature), and cause cataracts and neurological damage in suckling mice. The best studied species of spiroplasmas are Spiroplasma citri, the causative agent of Citrus Stuborn Disease, and Spiroplasma kunkelii, the causative agent of Corn Stunt Disease.
There is some disputed evidence for the role of spiroplasmas in the etiology of Transmissible Spongiform Encephalopathies (TSEs), due primarily to the work of Dr. Bastian, summarized below. Other researchers, such as Leach [1] have failed to replicate this work, while the prion model for TSEs has gained very wide acceptance. The most recent work of Alexeeva [2] appears to refute the role of spiroplasmas in the best small animal scrapie model (hamsters).
Bastian says:
- "spiroplasmas contain internal fibrillar proteins, that have morphological and immunological similarities to scrapie- and CJD-related fibrillar proteins. This comparison is noteworthy since mycoplasmologists consider these fibril proteins unique to this prokaryote.
- In vivo and in vitro experimental Spiroplasma infections produce cytopathic effects similar to those of the scrapie agent. Experimental Spiroplasma brain infection in the suckling rat is characterized by vacuolar encephalopathy with localization of the microbe to gray matter.
- ...Spiralins are chemically bound to Spiroplasma-associated fibrils (SpFs) and are separated with difficulty.' SpFs are unique internal fibrils of spiroplasmas with a molecular weight of 55 kDa. Recently, SpFs have been shown to bear close morphological resemblance to scrapie-associated fibrils (SAFS), ' and show cross-reactivity using SAF antibody." [3]
[edit] References
- ^ Creutzfeldt-Jakob disease. Failure to detect spiroplasmas by cultivation and serological tests: Leach, R. H., Mathews, W. B., and Will, R., J Neurol Sci 59(3): 349-53, 1983.
- ^ Absence of Spiroplasma or Other Bacterial 16S rRNA Genes in Brain Tissue of Hamsters with Scrapie. Alexeeva I, Elliott EJ, Rollins S, Gasparich GE, Lazar J, Rohwer RG. J Clin Microbiol. 2006 Jan;44(1):91-7. PMID: 16390954
- ^ Review of Theories on the Nature of the Transmissible Agent, Frank O. Bastian, M.D.
